HPLC Determination of Carbaryl on Newcrom B Column
https://sielc.com/hplc-determination-of-carbaryl-on-newcrom-b-columnChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Carbaryl .
Carbaryl , also known as Sevin and 1-naphthyl methylcarbamate, is an insecticide that works as a cholinesterase inhibitor. Carbaryl is highly toxic to zooplankton and works on both targeted and beneficial insects. It is classified as a potential human carcinogen.
Carbaryl can be retained on Newcrom B mixed-mode column in HPLC analysis with an isocratic method by using a mobile phase of acetonitrile (ACN) and water with ammonium formate (AmFm) as buffer, making the analytical method MS-compatible. Can also be UV detected at 280nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 280 nm |
| Class of Compounds | Hydrophobic, Herbicide, Pesticide |
| Analyzing Compounds | Carbaryl |
HPLC Column Used
Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended