HPLC Determination of Caffeine, Theophylline and Theobromine in Tea on Primesep SB Column
https://sielc.com/hplc-determination-of-caffeine-theophylline-and-theobromine-in-tea-on-primesep-sb-columnChromatogram
Method Parameters
| Mobile Phase | EtOH – 10% |
|---|---|
| Buffer | H3PO4 – 0.3% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Xanthine, Hydrophobic, Ionizable |
| Analyzing Compounds | Theophylline,Theobromine,Caffeine |
HPLC Column Used
Primesep SB, 3.2 x 100 mm, 5 µm, 100 A, dual ended