HPLC Method for Analysis of Bromhexine on Primesep 200 Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Bromhexine , Bromhexine hydrochloride .
Bromhexine is a popular mucolytic with the chemical formula C 14 H 20 Br 2 N 2 . It is used to treat excessive mucus found in the respiratory system from a variety of illnesses. It works through increasing the production of serous mucus, which thins phlegm. This helps transport phlegm out of the upper respiratory tract. You can find detailed UV spectra of Bromhexine and information about its various lambda maxima by visiting the following link.
Bromhexine , Bromhexine hydrochloride can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 60/40% |
|---|---|
| Buffer | H3PO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210, 245, 315 nm |
| Peak Retention Time | 2.81, 4.22 min |
| Class of Compounds | Drugs |
| Analyzing Compounds | Bromhexine,Bromhexine hydrochloride |