HPLC Method for Separation of Bromhexine and Ambroxol on Primesep 200 Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Bromhexine , Bromhexine hydrochloride , Ambroxol Hydrochloride , Ambroxol .
Ambroxol is a well-known expectorant with the chemical formula C 13 H 18 Br 2 N 2 O . It targets and breaks down phlegm through inhibiting neuronal Na+ channels. It also has anti-inflammatory properties. It has not yet been approved for use by the USA Food and Drug Administration. You can find detailed UV spectra of Ambroxol and information about its various lambda maxima by visiting the following link.
Bromhexine is a popular mucolytic with the chemical formula C 14 H 20 Br 2 N 2 . It is used to treat excessive mucus found in the respiratory system from a variety of illnesses. It works through increasing the production of serous mucus, which thins phlegm. This helps transport phlegm out of the upper respiratory tract. You can find detailed UV spectra of Bromhexine and information about its various lambda maxima by visiting the following link.
Bromhexine , Bromhexine hydrochloride , Ambroxol Hydrochloride , Ambroxol can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. This analysis method can be detected in the low UV regime at 210 nm, 245 nm, and 315 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 60/40% |
|---|---|
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210, 245, 315 nm |
| Peak Retention Time | 2.81, 4.22 min |
| Class of Compounds | Drugs |
| Analyzing Compounds | Bromhexine,Bromhexine hydrochloride,Ambroxol Hydrochloride,Ambroxol |