HPLC Method for Analysis of Brilliant Green on Primesep 100 Column
https://sielc.com/hplc-determination-of-brilliant-greenChromatogram
Description
· HPLC Method for Analysis of Brilliant Green on Primesep 100 Column by SIELC Technologies
Brilliant green , also known as zelyonka and zelenka , is a triarylmethane dye with the chemical formula C 27 H 33 N 2 .HO 4 S . A dilute alcoholic solution of Brilliant Green is used as a topical antiseptic. At lower solutions, it can also be used for mucous membranes and for infants. It is a visible light-activated photocatalyst in organic synthesis
Brilliant Green can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 600 nm with high resolution and peak symmetry.
Method Parameters
| Mobile Phase | MeCN/H2O – 80/20% |
|---|---|
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 600 nm |
| Peak Retention Time | 7.22 min |
| Class of Compounds | Dyes |
| Analyzing Compounds | Brilliant Green |