HPLC Method for Analysis of Butylated Hydroxytoluene on Newcrom R1 Column
https://sielc.com/hplc-determination-of-bhtChromatogram
Description
· HPLC Method for Analysis of Butylated hydroxytoluene on Newcrom R1 Column by SIELC Technologies
Butylated hydroxytoluene ( BHT ) is a synthetic antioxidant with the chemical formula C 15 H 24 O . It is widely used in a variety of industries, particularly in food preservation, cosmetics, and industrial products. It works by preventing oxidative degradation. While considered safe for use within regulatory limits, its potential health and environmental effects continue to be areas of study and discussion. You can find detailed UV spectra of Butylated hydroxytoluene and information about its various lambda maxima by visiting the following link.
Butylated hydroxytoluene can be retained, and analyzed on a mixed-mode Newcrom R1 column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid. This analytical method can be detected with high resolution and peak symmetry at a wavelength of 280 nm using UV detection.
Method Parameters
| Mobile Phase | MeCN/H2O – 80%, |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 280 nm |
| Injection volume | 2 µl |
| Sample | 1 mg/ml in MeCN/H2O – 80/20% |
| LOD* | 8 ppb |
| Class of Compounds | Diphenyl ethers |
| Analyzing Compounds | Butylated hydroxytoluene |