HPLC Determination of Benzoic Anhydride on Newcrom R1 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzoic Anhydride.
Benzoic Anhydride is the more active analogue of benzoic acid formed via dehydration. It has the chemical formula C 14 H 10 O 3 . Benzoic anhydride is used as a benzoylating agent in the manufacture of pharmaceuticals, intermediates and dyes. It is considered the simplest symmetrical aromatic acid anhydride.
Benzoic Anhydride can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN), water and detected by UV, ELSD, CAD or LC/MS.
Method Parameters
| Mobile Phase | MeCN – 70% |
|---|---|
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Hydrophobic |
| Analyzing Compounds | Benzoic anhydride |
HPLC Column Used
Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended