HPLC Method for Analysis of Benzalkonium Chloride on Primesep SB Column
https://sielc.com/hplc-determination-of-benzalkoniumChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode · Method for Analysis of Benzalkonium chloride on Primesep SB by SIELC Technologies
Benzalkonium chloride, also known as Zephiran or Alkyldimethylbenzylammonium chloride, is a positively charged surfactant often used as a pharmaceutical preservative and as an antiseptic. Benzalkonium Chloride can be retained on a mixed-mode stationary phase Primesep SB column with embedded strong basic ion-pairing groups, using a gradient analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) ionic modifier. This analysis method can be UV detected at 210 nm with high resolution and peak symmetry.
Method Parameters
| Mobile Phase | Gradient MeCN – 30-70%, 10 min |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 210 nm |
| Peak Retention Time | 5.32, 6.93 |
| Class of Compounds | Drug |
| Analyzing Compounds | Benzalkonium chloride |
HPLC Column Used
Primesep SB, 3.2 x 100 mm, 5 µm, 100 A, dual ended