HPLC Determination of Azithromycin on Primesep SB Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Azithromycin .
Azithromycin is an antibiotic used in the treatment of various bacterial infections. It is on the WHOs list of essential medicines and is classified as critically important. In reversed-phase HPLC, it generally exhibits significant tailing. The tailing can be substantially reduced by using a mixed-mode Primesep SB column with a simple MS-compatible mobile phase of water, acetonitrile (ACN) and ammonium formate (AmFm) buffer.
Method Parameters
| Mobile Phase | MeCN/H2O – 15/85% |
|---|---|
| Buffer | AmFm pH 3.0 – 50 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Drug, Antibiotics |
| Analyzing Compounds | Azithromycin |
HPLC Column Used
Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended