HPLC Determination of Atrolactic Acid on Primesep 200 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Atrolactic Acid .
Atrolactic Acid is a 2-hydroxy monocarboxylic acid with the chemical formula C 9 H 10 O 3 . It can cause irritation to eyes, skin, and the respiratory system.
Atrolactic Acid can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Drug, Acid, Hydrophobic |
| Analyzing Compounds | Atrolactic Acid |
HPLC Column Used
Primesep 200, 4.6 x 150 mm, 3 µm, 100 A, dual ended