HPLC-MS Method for Analysis of Arbidol (Umifenovir) on Primesep 100 Column

Chromatogram

Description

· HPLC Method for Analysis of Arbidol (Umifenovir) on Primesep 100 Column by SIELC Technologies

Umifenovir is an antiviral medication with the chemical formula C 22 H 25 BrN 2 O 3 S . It has been used to treat influenza in Russia and China for decades, but it is not approved for use in the United States by the Food and Drug Administration (FDA).  It is considered a direct-acting antiviral as well as a host-targeting agent due to how it effects multiple stages of a viral lifecycle. Due to it’s wide-spread capabilities in covering both enveloped and non-enveloped RNA and DNA viruses, it is currently being investigated as treatment and prevention of COVID-19 caused by SARS-CoV-2 infections.

Mechanism of Action : Umifenovir interferes with the viral ability to penetrate into host cells. It acts by inhibiting the fusion of the viral lipid membrane with the host cell membrane, thus preventing the virus from entering and infecting the cell.

Uses :

Side Effects : Though generally considered to be safe, some reported side effects of Umifenovir include digestive disturbances, allergic reactions, or elevated heart rate. However, side effects are generally rare. As always, any medication’s potential side effects should be discussed with a healthcare provider.

You can find detailed UV spectra of Arbidol (Umifenovir) and information about its various lambda maxima by visiting the following link.

Arbidol (Umifenovir) can be retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Ammonium formate as a buffer. This analysis method can be detected using UV at 254 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)

Method Parameters

HPLC Column Used