HPLC Determination of Allantoin on SHARC 1 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Allantoin .
Allantoin is a 5-ureidohydantoin or glyoxyldiureide with the chemical formula C 4 H 6 N 4 O 3 . It is s metabolic intermediate that is found in plants like chamomile, wheat sprouts, tobacco seed, sugar beet and comfrey. It is frequently used in cosmetics and hygiene products.
Allantoin can be retained using HPLC on SHARC 1 column, which uses hydrogen-bonding as a separation mechanism with isocratic method by using a mobile phase of acetonitrile (ACN) and methanol (MeOH). Allantoin can be monitored by low UV (210 nm), ELSD, CAD or LC/MS.
Method Parameters
| Mobile Phase | MeCN/MeOH – 90/10% |
|---|---|
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Hydrophilic, Amide, Amine |
| Analyzing Compounds | Allantoin |
HPLC Column Used
SHARC 1, 4.6 x 150 mm, 5 µm, 100 A, dual ended