HPLC Separation of Alizarin and Alizarin Red S on Newcrom BH Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Alizarin , Alizarin Red S .
Alizarin and Alizarin Red S are both dyes with applications in locating calcium deposits in tissues. They differ in Alizarin Red S having an additional sulfonic acid group. They can be separated in HPLC using a Newcrom BH mixed-mode column using a mobile phase of methanol (MeOH) and water and sulfuric acid (H2SO4) as a buffer. UV detection at 250nm.
Method Parameters
| Mobile Phase | MeOH/H2O – 75/25% |
|---|---|
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Class of Compounds | Acid, Dyes |
| Analyzing Compounds | Alizarin,Alizarin Red S |
HPLC Column Used
Newcrom BH, 4.6 x 50 mm, 5 µm, 100 A, dual ended