HPLC Method for Separation Alexidine and Polyhexanide on BIST B+ Column

Chromatogram

Description

· HPLC Method for Analysis of Polyhexanide PHMB , Alexidine on on BIST B+ by SIELC Technologies

Alexidine is a chemical compound that belongs to the class of bis-biguanide antiseptics and has the chemical formula C 26 H 56 N 10 . It is known for its antimicrobial properties and is used in various products for disinfection and antiseptic purposes. Alexidine has broad-spectrum antimicrobial activity, and it is commonly used in healthcare settings for its effectiveness against bacteria, fungi, and some viruses. In some formulations, alexidine is used as a topical antiseptic to disinfect skin and mucous membranes. It is found in some mouthwashes, oral care products, contact lens solutions, and more It may be included in certain wound care products for its ability to prevent infection. You can find detailed UV spectra of Alexidine and information about its various lambda maxima by visiting the following link.

Polyhexanide ( polyhexamethylene biguanide , PHMB ) is a disinfectant with the chemical formula (C 8 H 17 N 5 ) n . It has multiple industrial, medical, biological and research applications especially for materials that could be a possible source of bacterial contamination. Dermatologically, it is sold as polihexanide. From 2011, it has been classified as a category 2 carcinogen. You can find detailed UV spectra of Polyhexanide PHMB and information about its various lambda maxima by visiting the following link.

Polyhexanide PHMB , Alexidine can be retained, separated and analyzed on a BIST B+ mixed-mode stationary phase column using an analytical method with a simple mobile phase of water, Acetonitrile (MeCN) , and a sulfuric acid as a buffer. This analysis method can be detected using UV at 200 nm.

Method Parameters

HPLC Column Used