HPLC Method for Analysis of 4-(2-Aminoethyl) benzenesulfonamide on Primesep 100 Column
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Description
4-(2-Aminoethyl) benzenesulfonamide is an amide with the chemical formula C 8 H 12 N 2 O 2 S . When it is combined with hypoxic conditions, can inhibit the growth and induce apoptosis of human hepatocellular carcinoma cells. It is commonly used as an intermediate in synthesis of antidiabetic drugs. You can find detailed UV spectra of 4-(2-Aminoethyl)benzenesulfonamide and information about its various lambda maxima by visiting the following link.
4-(2-Aminoethyl)benzenesulfonamide can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) buffer. This analysis method can be detected in the low UV regime at 220 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 220, 265 nm |
| Peak Retention Time | 5.21 min |
| Class of Compounds | Amide |
| Analyzing Compounds | 4-(2-Aminoethyl)benzenesulfonamide |