HPLC Determination of Acid red 1 on Newcrom BH Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of C.I. Acid Red 1 .
Acid Red 1, also known as Red 2G, is a synthetic dye that’s used as a food additive. It has been banned in several countries due to safety concerns because it forms aniline in the body that can interfere with the function of hemoglobin. Acid Red 1 can be retained on HPLC mixed-mode Newcrom BH column using a mobile phase consisting of methanol (MeOH) and water with sulfuric acid (H2SO) buffer. Vis detection at 535nm.
Method Parameters
| Mobile Phase | MeOH/H2O – 75/25% |
|---|---|
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | Vis 535 nm |
| Class of Compounds | Acid, Dyes |
| Analyzing Compounds | C.I. Acid Red 1 |
HPLC Column Used
Newcrom BH, 4.6 x 50 mm, 5 µm, 100 A, dual ended