HPLC Determination of 6-methyl-5-hepten-2-one on Newcrom R1 Column

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Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Methyl-5-hepten-2-one .

Sulcatone is a ketone used as a mosquito attractant. It is a volatile oil component found in citrus. It can be retained on a Newcrom R1reverse-phase column with a mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer. The analytical method is MS-compatible and can also use UV detection at 200nm.

Method Parameters

Mobile PhaseMeCN/H2O – 50/50%
BufferH2SO4 – 0.1 %
Flow Rate1.0 ml/min
DetectionUV, 200 nm
Class of CompoundsHydrophobic, Mosquito attractant
Analyzing Compounds6-Methyl-5-hepten-2-one

HPLC Column Used