HPLC Determination of 6-Benzylaminopurine on Newcrom R1 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Benzylaminopurine .
6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C 12 H 11 N 5 . It’s presence in asparagus, for example, leads to deeper color, increased firmness, and a decrease in fibrous hardness. In general, it stimulates cell division and differentiation. In floristry it is used to extend the shelf life of cut flowers and greens. You can find detailed UV spectra of 6-Benzylaminopurine and information about its various lambda maxima by visiting the following link.
6-Benzylaminopurine can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | UV, 272 nm |
| Class of Compounds | Hydrophobic, Herbicide, Pesticide |
| Analyzing Compounds | 6-Benzylaminopurine |