HPLC Method for Separation of Acetaminophen and 4-Aminophenol on Primesep 100 Column
https://sielc.com/hplc-determination-of-4-aminophenol-acetaminophenChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode
4-Aminophenol (also known as para-aminophenol or Rodinal) is a popular film developing compound and building block in organic chemistry reactions. Most notably, it is a key compound used in the production of acetaminophen (also known as paracetamol or Tylenol). 4-Aminophenol can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an sulfuric acid (H2SO4) or perchloric acid (HClO4) buffer. This analysis method can be detected in the UV regime at 220 nm.
Method Parameters
| Mobile Phase | MeCN – 10% |
|---|---|
| Buffer | H2SO4 – 0.2% or HClO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 220 nm |
| Peak Retention Time | 4.25, 5.51 min |
| Class of Compounds | Alcohols, Phenols, Amines, Amine Salts, Dgru |
| Analyzing Compounds | 4-Aminophenol, Acetaminophen |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended