HPLC Determination of 3-Iodo-2-Propynyl Butylcarbamate on Newcrom R1 Column

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Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of 3-Iodo-2-propynyl-N-butylcarbamate .

Iodopropynyl Butyl Carbamate is a water-based preservative used in paints, coating, personal care and cosmetic industries. It is used because of its fungicide properties. It can be retained on Newcrom R1 reverse-phase column using an isocratic analytical method using a mobile phase of acetonitrile (ACN) and water with a phosphoric acid (H3PO4) buffer with UV detection at 200nm.

Method Parameters

Mobile PhaseMeCN/H2O – 50/50%
BufferAmFm pH 3.0- 10 mM
Flow Rate1.0 ml/min
DetectionUV, 200 nm
Class of CompoundsPreservative, Hydrophobic, Ionizable, Zwitterionic
Analyzing Compounds3-Iodo-2-propynyl-N-butylcarbamate

HPLC Column Used

Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended