HPLC Determination of 1,5-Naphthalenedisulfonic Acid on Newcrom B Column

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Chromatogram

Method Parameters

Mobile PhaseMeCN/H2O – 70/30%
BufferAmmonium Formate pH 3.0 – 40 mM
Flow Rate0.5 ml/min
DetectionUV 285 nm
Class of CompoundsAcid
Analyzing Compounds1,5-Naphthalenedisulfonic Acid

HPLC Column Used

Newcrom B, 3.2 x 100 mm, 5 µm, 100 A, dual ended
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