HPLC Determination of 1-pyridin-3-yl-ethylamine on Primesep 100 Column
https://sielc.com/hplc-determination-of-1-pyridin-3-yl-ethylamine-on-primesep-100-columnChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of 1-pyridin-3-yl-ethylamine. · 1-pyridin-3-yl-ethylamine has the chemical formula C 7 H 10 N 2 .
1-pyridin-3-yl-ethylamine can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 260 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | H2SO4 – 0.3% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 260 nm |
| Class of Compounds | Amine |
| Analyzing Compounds | 1-pyridin-3-yl-ethylamine |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended