HPLC Determination of 1-(2-Methylphenyl)-1-pyridin-4-ylmethanamine dihydrochloride on Primesep 100 Column
https://sielc.com/hplc-determination-of-1-2-methylphenyl-1-pyridin-4-ylmethanamine-dihydrochloride-on-primesep-100-columnChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of 1-(2-Methylphenyl)-1-pyridin-4-ylmethanamine dihydrochloride
1-(2-Methylphenyl)-1-pyridin-4-ylmethanamine dihydrochloride has the chemical formula C 13 H 14 ClN 2 – .
You can find detailed UV spectra of 1-(2-Methylphenyl)-1-pyridin-4-ylmethanamine dihydrochloride and information about its various lambda maxima by visiting the following link.
1-(2-Methylphenyl)-1-pyridin-4-ylmethanamine dihydrochloride can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | H2SO4 – 0.3% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Amine |
| Analyzing Compounds | 1-(2-Methylphenyl)-1-pyridin-4-ylmethanamine dihydrochloride |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended