HPLC Determination of Δ8-THC, CBD, Δ9-THC, and CBN on Primesep N Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Cannabidiol (CBD) , Cannabinol (CBN) , Delta-8-Tetrahydrocannabinol (8-THC) , Delta-9-Tetrahydrocannabinol (THC) .
Δ8-THC , Δ9-THC , CBD and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. THC has psychoactive effects, while CBD does not.
They can be separated using HPLC on a normal-phase Primesep N column using a mobile phase consisting of hexane and ethyl acetate without the need for a buffer with UV detection at 275nm.
Method Parameters
| Mobile Phase | Hexane/Ethyl Acetate – 97/3% |
|---|---|
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 275 nm |
| Class of Compounds | Drug, Hydrophilic, Ionizable, Supplements |
| Analyzing Compounds | Cannabidiol (CBD),Cannabinol (CBN),Delta-8-Tetrahydrocannabinol (8-THC),Delta-9-Tetrahydrocannabinol (THC) |
HPLC Column Used
Primesep N, 4.6 x 150 mm, 3 µm, 100 A, dual ended