HPLC Determination of Polyhexanide (PHMB) on PEI Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Polyhexanide PHMB
Polyhexanide (polyhexamethylene biguanide, PHMB) is a polymer used as a disinfectant and antiseptic. We estimated that limit of detection is about 2 ppm for this compounds with this method.
The peak of polyhexanide fractionated on several peaks due to difference in length of the polymer components.
Method Parameters
| Mobile Phase | MeCN/H2O – 40/60% |
|---|---|
| Buffer | AmFm pH 3.0- 20 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 236 nm |
| Class of Compounds | Polymer, Hydrophilic, Ionizable, Antiseptic, Disinfectant |
| Analyzing Compounds | Polyhexanide PHMB |
HPLC Column Used
PEI, 4.6 x 250 mm, 5 µm, 100 A, dual ended