HPLC Determination of Dicyandiamide on Sharc 1 Column
https://sielc.com/hplc-determenation-of-dicyandiamideChromatogram
HPLC Determenation of Dicyandiamide on Sharc 1 Column_1200
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Dicyandiamide
Dicyandiamide, also known as cyanoguanidine, has a C₂H₄N₄ chemical formula. It is a white crystalline powder that has a variety of uses from agricultural to mechanical. Though, typically it is found in fertilizers as a nitrification inhibitor. It slows down nitrogen release and and reduces losses from soil.
Dicyandiamide can be retained and analyzed using the SHARC 1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a triethanolamine buffer. Detection is performed using CAD.
Method Parameters
| Mobile Phase | MeCN – 97.8% |
|---|---|
| Buffer | AmFm 0.2%, Formic Acid – 2% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) MS- compatible mobile phase |
| Class of Compounds | Amide, Hydrophilic, Ionizable |
| Analyzing Compounds | Dicyandiamide |
HPLC Column Used
SHARC 1, 4.6 x 150 mm, 5 µm, 100 A, dual ended