HPLC Chiral Separation of Verapamil Enantiomers on Cyralis CM Column

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Verapamil .

Verapamil is a medication with the chemical formula C 27 H 28 N 2 O 4 . It is a calcium channel blocker that is used primarily to treat high blood pressure, angina, and supraventricular tachycardia. On occasion, it is used to prevent migraines and cluster headaches. You can find detailed UV spectra of Verapamil and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Verapamil enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.

Verapamil can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of AmAC and MeCN. Detection is performed using UV.

Method Parameters

HPLC Column Used