HPLC Chiral Separation of Pantoprazole Enantiomers on Cyralis CL Column

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Pantoprazole .

Pantoprazole is a proton pump inhibitor (PPI) with C 16 H 15 F 2 N 3 O 4 S molecular formula. It is used to decrease stomach acids amounts. It does that through binding to proton pumps in the stomach’s lining, therefore preventing the production of stomach acid. You can find detailed UV spectra of Pantoprazole and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Pantoprazole enantiomers using SIELC’s Cyralis CL chiral column. Cyralis CL is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Pantoprazole can be retained and analyzed using the Cyralis CL stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of sodium perchlorate and acetonitrile. Detection is performed using UV.

Method Parameters

HPLC Column Used