HPLC Chiral Separation of Trans-Stilbene Oxide on Cyralis CM Column

https://sielc.com/hplc-chiral-method-for-analysis-of-trans-stilbene-oxide
⬇ PDF Download

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Trans-Stilbene Oxide .

Trans-Stilbene Oxide is a an organic compound with the chemical formula C 14 H 12 O . It is used primarily in photochemistry and as a stationary phase modifier in Chiral Chromatography You can find detailed UV spectra of Trans-Stilbene Oxide and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Trans-Stilbene Oxide enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Trans-Stilbene Oxide can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane and Ethanol. Detection is performed using UV.

Method Parameters

Mobile PhaseHexane/Ethanol- 90/10%
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution3.5
Class of CompoundsOrganic Compound
Analyzing CompoundsTrans-Stilbene Oxide

HPLC Column Used

Cyralis CM, 4.6 x 100 mm, 5 µm, 100 A, dual ended