HPLC Chiral Separation of Thalidomide Enantiomers on Cyralis CM Column

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Thalidomide .

Thalidomide is a an medication with the chemical formula C 13 H 10 N 2 O 4 . It is primarily used to treat cancer, graft-versus-host disease, and various skin disorders. Thalidomide can cause birth defects, which was a fact originally unknown when it entered the German market in 1957. Over 10,000 children were harmed by Thalidomide, which has led to stricter drug regulation and monitoring in many countries.

This application describes a normal-phase chiral HPLC method for the separation of Thalidomide enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Thalidomide can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane and IPA. Detection is performed using UV.

Method Parameters

HPLC Column Used