HPLC Chiral Separation of Paclobutrazol Enantiomers on Cyralis AM Column

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Paclobutrazol .

Paclobutrazol (PBZ) is an organic fungicide with the chemical formula C 15 H 20 ClN 3 O . It works through inhibiting the production of gibberellins, which are plant growth hormones. Through reducing reducing internodal growth, it causes stronger stems, greater root growth, and earlier fruitset. It can also reduce frost sensitivity in plants. You can find detailed UV spectra of Paclobutrazol and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Paclobutrazol enantiomers using SIELC’s Cyralis AM chiral column. Cyralis AM is an amylose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Paclobutrazol can be retained and analyzed using the Cyralis AM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and TFA. Detection is performed using UV.

Method Parameters

HPLC Column Used