HPLC Chiral Separation of Omeprazole Enantiomers on Cyralis CL Column
https://sielc.com/hplc-chiral-method-for-analysis-of-omeprazoleChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Omeprazole .
Omeprazole is a proton pump inhibitor (PPI) with the chemical formula C 17 H 19 N 3 O 3 S . It is a medication that decreases the amount of acid produced in the stomach. Omeprazole has a bactericidal effect on Helicobacter pylori.
This application describes a normal-phase chiral HPLC method for the separation of Omeprazole enantiomers using SIELC’s Cyralis CL chiral column. Cyralis CL is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.
Omeprazole can be retained and analyzed using the Cyralis CL stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of sodium perchlorate and acetonitrile. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/100 mM Ammonium Carbonate pH 9 |
|---|---|
| Flow Rate | 1.0 mL/min |
| Detection | UV 280 nm |
| Resolution | 2.14 |
| Class of Compounds | Proton Pump Inhibitor |
| Analyzing Compounds | Omeprazole |