HPLC Chiral Separation of Nebivolol Enantiomers on Cyralis AM Column

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Nebivolol .

Nebivolol is a beta-blocker with the chemical formula C 22 H 25 F 2 NO 4 . It is an oral medication used to treat high blood pressure, but is not preferred to alternatives. Side effects of it include dizziness, fatigue, nausea, and headaches. You can find detailed UV spectra of Nebivolol and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Nebivolol enantiomers using SIELC’s Cyralis AM chiral column. Cyralis AM is an amylose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Nebivolol can be retained and analyzed using the Cyralis AM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and DEA. Detection is performed using UV.

Method Parameters

HPLC Column Used