HPLC Chiral Separation of Mirtazapine Enantiomers on Cyralis CM Column

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Mirtazapine .

Mirtazapine is an atypical tetracyclic antidepressant with the chemical formula C 17 H 19 N 3 . It is used primarily to treat depression that is complicated by anxiety or insomnia.

This application describes a normal-phase chiral HPLC method for the separation of Mirtazapine enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.

Mirtazapine can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of sodium Hexane, Ethanol, and DEA. Detection is performed using UV.

Method Parameters

HPLC Column Used