HPLC Chiral Separation of Doxazosin Enantiomers on Cyralis CM Column

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Doxazosin .

Doxazosin is a medication with the chemical formula C 23 H 25 N 5 O 5 . It is used to treat symptoms of of post-traumatic stress disorder, hypertension, and benign prostatic hyperplasia. It works as an alpha-1 blocker and a 1 -adrenergic receptor antagonist.

This application describes a normal-phase chiral HPLC method for the separation of Doxazosin enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.

Doxazosin can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and 2-(dimethylamino)ethanol. Detection is performed using UV.

Method Parameters

HPLC Column Used