HPLC Chiral Separation of Bromadiolone Enantiomers on Cyralis CM Column

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Bromadiolone .

Bromadiolone is a an anticoagulant with the chemical formula C 30 H 23 BrO 4 . It works as a rodenticide which has a tendency to accumulate in the liver of the poisoned organism. It is a second-generation 4-hydroxycoumarin derivative. Vitamin K1 is considered to be an antidote to the pesticide. You can find detailed UV spectra of Bromadiolone and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Bromadiolone enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.

Bromadiolone can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and TFA. Detection is performed using UV.

Method Parameters

HPLC Column Used