HPLC Chiral Separation of Bromacil Enantiomers on Cyralis CM Column

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Bromacil .

Bromacil is a an organic compound with the chemical formula C 9 H 13 BrN 2 O 2 . It is used primarily as a pesticide and herbicide commercially. It works through interfering with photosynthesis. On a limited number of crops, it can be used as a selective weed control, but it is mostly nonselective and broad spectrum. It is toxic through oral, dermal, and inhalation routes and is in Toxicity Category IV. You can find detailed UV spectra of Bromacil and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Bromacil enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Bromacil can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane and IPA. Detection is performed using UV.

Method Parameters

HPLC Column Used