HPLC Method for Analysis of Tylosin A, B, C, D on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Tylosin
Tylosin A ( C 46 H 77 NO 1 7 ), Tylosin B ( C 39 H 65 NO 14 ), Tylosin C ( C 45 H 75 NO 17 ), and Tylosin D (C 46 H 79 NO 17 ) are antibiotics that are typically used to treat infections and inflammations, especially in veterinary cases.
Tylosin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using ELSD.
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Method Parameters
| Mobile Phase | Gradient MeCN/H2O from 10/90% to 60/40% 30 min |
|---|---|
| Buffer | Ammonium Formate pH 3.0 – 60 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 286 |
| Class of Compounds | Antibiotic |
| Analyzing Compounds | Tylosin |