HPLC MS Method for Analysis of Tetramisole on Primesep 200 Column
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Description
· HPLC Method for Analysis of (+-)-Tetramisole in on Primesep 200 Column
Tetramisole is a medication with the chemical formula C 11 H 12 N 2 S . It is primarily used to treat and get rid of parasitic worms in animals. It works through inhibiting alkalinephosphates, which freezes muscles of the worm. You can find detailed UV spectra of (+-)-Tetramisole and information about its various lambda maxima by visiting the following link.
(+-)-Tetramisole can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of acetonitrile, water, and ammonium formate. Detection is performed using MS.
Method Parameters
| Mobile Phase | MeCN/H2O -40/60% |
|---|---|
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 0.5 ml/min |
| Detection | ESI-SIM: [M-H]–m/z 205 |
| Class of Compounds | Drugs |
| Analyzing Compounds | (+-)-Tetramisole |
HPLC Column Used
Primesep 200, 3.2 x 150 mm, 5 µm, 100 A, dual ended