HPLC MS Method for Analysis of Phosphonoacetic Acid on Chromni Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Phosphonoacetic acid
Phosphonoacetic Acid , also written as PAA , is an organophosphorus compound with the chemical formula C 2 H 5 O 5 P . It is used primarily in research as a targeted inhibitor of DNA polymerase in herpesviruses, cytomegalovirus, and orthopoxyviruses. It is also a structural precursor and model in broad-spectrum antiviral agents.
Phosphonoacetic acid can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an sulfuric acid as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 75/25% |
|---|---|
| Buffer | H2SO4– 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Phosphonoacetic acid |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated