HPLC Method for Analysis of Myelin Basic Protein (85-99) Peptide Antagonist on Chromni Column
https://sielc.com/hplc-analysis-of-myelin-basic-protein-85-99-peptide-antagonistChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Myelin Basic Protein Peptide Antagonist
Myelin Basic Protein Peptide Antagonist is a synthetic peptide antagonist with the molecular formula C 70 H 114 N 18 O 21 • XCF 3 COOH . When expressing an MBP (85-99), it inhibits the production of IL-2 in T cell hybridoma cells. MBP peptide antagonist also delays MBP (85-99)-induced experimental autoimmune encephalomyelitis and reduces symptom severity. You can find detailed UV spectra of Myelin Basic Protein Peptide Antagonist and information about its various lambda maxima by visiting the following link.
Myelin Basic Protein Peptide Antagonist is analyzed using a Chromni stationary phase column under gradient conditions with a mobile phase composed of acetonitrile (MeCN) and water. Detection is performed by UV absorbance detection.
Method Parameters
| Mobile Phase | MeCN/H2O- 80/20 – 60/40%, 10 min |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm, UV 280 nm |
| Limit of Detection* | 200 nm – 46 ppb, 280 nm – 733.9 ppb |
| Class of Compounds | Peptide |
| Analyzing Compounds | Myelin Basic Protein Peptide Antagonist |