HPLC Method for Analysis of Insulin Glargine on Chromni Column
https://sielc.com/hplc-analysis-of-insulin-glargineChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Insulin Glargine
Insulin Glargine is a modified form of medical insulin with the molecular formula C 267 H 404 N 72 O 78 S 6 . It is used in management of type 1 and type 2 diabetes. Insulin glargine differs from human insulin by replacing asparagine with glycine in position 21 of the A-chain. The patent within the European Union and the United States in 2014.
Insulin Glargine is analyzed using a Chromni stationary phase column under gradient conditions with a mobile phase composed of acetonitrile (MeCN) and water. Detection is performed by UV absorbance detection.
Method Parameters
| Mobile Phase | MeCN/H2O- 80/20 – 60/40%, 10 min |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm, UV 280 nm |
| Limit of Detection* | 200 nm – 54.8 ppb, 280 nm – 770.6 ppb |
| Class of Compounds | Medication |
| Analyzing Compounds | Insulin Glargine |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated