HPLC Method for Analysis of Donepezil on Primesep 100 Column
https://sielc.com/hplc-analysis-of-donepezil-on-primesep-100Chromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Donepezil
Donepezil is a medication with the chemical formula C 24 H 29 NO 3 . It is primarily used to treat dementia and improve cognition, but has not shown to cause any change in the progression of the disease. Some research suggests that it can be used to treat Lewy body dementia, effects of traumatic brain injury, and vascular dementia. You can find detailed UV spectra of Donepezil and information about its various lambda maxima by visiting the following link.
Donepezil can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | Sulfuric Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV 208 nm |
| Limit of Detection* | 16 ppb |
| Class of Compounds | Drug |
| Analyzing Compounds | Donepezil |