HPLC Method for Analysis of Cyclosporine H on Legacy L16 Column
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Description
· HPLC Method for Analysis of Cyclosporin H in on Legacy L16 Column
Cyclosporine H is a selective inhibitor of formyl peptide receptor-1 (FPR-1) with the molecular formula C 61 H 111 N 11 O 12 . It is used primarily used in research. Unlike Cyclosporin A, B, and C, it does not have immunosuppressive properties. You can find detailed UV spectra of Cyclosporine H and information about its various lambda maxima by visiting the following link .
Cyclosporin H can be retained and analyzed using the Legacy L16 stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of acetonitrile, water, methanol, and phosphoric acid. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/MeOH/H2O – 50/5/45% |
|---|---|
| Buffer | H3PO4– 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 |
| Column Temperature | 70 C |
| Class of Compounds | Inhibitor |
| Analyzing Compounds | Cyclosporin H |
HPLC Column Used
Legacy L16, 4.6 x 150 mm, 5 µm, 100 A, dual ended