HPLC Method for Analysis of Blasticidin S on BIST B Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Blasticidin-S
Blasticidin S is an antibiotic with the chemical formula C 17 H 26 N 8 O 5 . It is primarily used in biology research for selecting cells of interest among cell cultures. It works through disrupting protein translation within human cells, fungi, and bacteria due to it’s resemblance to cytidine. You can find detailed UV spectra of Blasticidin-S and information about its various lambda maxima by visiting the following link.
Blasticidin-S can be retained and analyzed using the BIST B stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | Gradient MeCN/H2O – 80/20 – 50/50 %, 10 min |
|---|---|
| Buffer | Sulfuric Acid – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275nm |
| Limit of Detection* | 13 ppb |
| Class of Compounds | Antibiotic |
| Analyzing Compounds | Blasticidin-S |
HPLC Column Used
BIST B, 4.6 x 50 mm, 5 µm, 100 A, dual ended