HPLC Method for Analysis of beta-Carotene on Lipak Column

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of beta-Carotene

beta-Carotene , also written as β-Carotene , is a pigment with the chemical formula C 40 H 56 . It is found primarily in fungi, plants, and fruit. Especially high amounts of it are found in carrots, pumpkins, spinach, and sweet potatoes. In dietary contexts, it is used as a pro-vitamin, as it converts to retinol in the body. In fact, it is the worldwide primary dietary source of provitamin A and is the best known provitamin a carotenoid. You can find detailed UV spectra of beta-Carotene and information about its various lambda maxima by visiting the following link.

beta-Carotene is analyzed using a Lipak stationary phase column under isocratic conditions with a mobile phase composed of acetonitrile (MeCN) and ethanol. Detection is performed by visible (Vis) absorbance detection

Method Parameters

HPLC Column Used