HPLC Method for Analysis of Ascorbic Acid on Primesep AP Column
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Description
· HPLC Method for Analysis of Ascorbic Acid in on Primesep AP Column
Ascorbic Acid is a vitamin with the molecular formula C 6 H 8 O 6 . Typically, it is used to treat scurvy, support immune system, and preserve food. It is a white to light yellow powder that is easily dissolved in water. It can be found in a large variety of fruits and vegetables, especially in citrus. You can find detailed UV spectra of Ascorbic Acid and information about its various lambda maxima by visiting the following link.
Ascorbic Acid can be retained and analyzed using the Primesep AP stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of acetonitrile, water, and ammonium acetate. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | AmAc pH 5.0 – 30 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV 275 nm |
| Limit of Detection* | 53.8 ppb |
| Class of Compounds | Vitamin |
| Analyzing Compounds | Ascorbic Acid |
HPLC Column Used
Primesep AP, 3.2 x 100 mm, 5 µm, 100 A, dual ended