HPLC Method for Analysis of Angiotensin II on Chromni Column
https://sielc.com/hplc-analysis-of-angiotensin-iiChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of (Compound not found)
Angiotensin II is a peptide hormone with the chemical formula C 50 H 71 N 13 O 12 . It is said to exhibit endocrine, autocrine/paracrine, and intracrine functions. Within the proximal tubules of the kidney, it regulates water and Na + reabsorption. Synthetic version of the peptide is used to treat hypotension caused by distributive shock.
(Compound not found) is analyzed using a Chromni stationary phase column under gradient conditions with a mobile phase composed of acetonitrile (MeCN) and water. Detection is performed by UV absorbance detection.
Method Parameters
| Mobile Phase | MeCN/H2O- 80/20 – 60/40% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm, UV 280 nm |
| Limit of Detection* | 200 nm – 27.5 ppb, 280 nm – 222.4 ppb |
| Class of Compounds | hormone |
| Analyzing Compounds | (Compound not found) |
HPLC Column Used
(Column variation not found)