HPLC Analysis of Bivalirudin on Promix MP colum
https://sielc.com/hplc%20analysis%20of%20bivalirudinChromatogram
Chr_200
Description
Bivalirudin is a short 21 amino acids peptide. It has 2 basic and 6 acidic groups. It is a naturally occurred an anticoagulant. Asp 9 – bivalirudin is a process impurity and can be a result of degradation of bivalirudin drug.
Method Parameters
| Mobile Phase | Gradient MeCN – 30-60%, 35 min |
|---|---|
| Buffer | Na2HPO4 pH 3.0- 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 215 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable, Blood thinners |
| Analyzing Compounds | Bivalirudin |
HPLC Column Used
Promix MP, 4.6×250 mm, 5 µm, 100A