Determination Pantoprazole on Obelisc R Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Pantoprazole , Pantoprazole sodium .
Pantoprazole is a proton pump inhibitor (PPI) with C 16 H 15 F 2 N 3 O 4 S molecular formula. It is used to decrease stomach acids amounts. It does that through binding to proton pumps in the stomach’s lining, therefore preventing the production of stomach acid.
Pantoprazole , Pantoprazole sodium can be retained on an Obelisc R column, which has both positive and negative ion-pairs embedded in the stationary phase, allowing for the fine tuning and separation of a wide range of compounds with different ionic properties. Pantoprazole can be determined isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer and detected by UV at 290nm, ELSD, CAD or LC/MS.
Method Parameters
| Mobile Phase | MeCN -35% |
|---|---|
| Buffer | Ammonium Acetate pH 5.5 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 290 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Pantoprazole,Pantoprazole sodium |